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p6010  (New England Biolabs)


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    Structured Review

    New England Biolabs p6010
    P6010, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 431 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p6010s/Casein+Kinase+II/pm41961893-225-15-12
    Average 96 stars, based on 431 article reviews
    p6010 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    In Vitro:

    Article Title: Regulation of FXR1 by alternative splicing is required for muscle development and controls liquid-like condensates in muscle cells
    Article Snippet: .. In vitro phosphorylation reactions were carried out according to the manufactures instructions (NEB, P6010S) using 0.9 nanomoles of protein with control phosphorylation performed in the absence of ATP with all other reaction components. ..

    Phospho-proteomics:

    Article Title: Regulation of FXR1 by alternative splicing is required for muscle development and controls liquid-like condensates in muscle cells
    Article Snippet: .. In vitro phosphorylation reactions were carried out according to the manufactures instructions (NEB, P6010S) using 0.9 nanomoles of protein with control phosphorylation performed in the absence of ATP with all other reaction components. ..

    Control:

    Article Title: Regulation of FXR1 by alternative splicing is required for muscle development and controls liquid-like condensates in muscle cells
    Article Snippet: .. In vitro phosphorylation reactions were carried out according to the manufactures instructions (NEB, P6010S) using 0.9 nanomoles of protein with control phosphorylation performed in the absence of ATP with all other reaction components. ..

    other:

    Article Title: PP2A licenses the FANCD2/FANCI complex for chromosome loading
    Article Snippet: CK2 , NEB , Cat# P6010S.

    Article Title: The Phospho-Code Determining Circadian Feedback Loop Closure and Output in Neurospora
    Article Snippet: Casein Kinase II (CK2) , New England Biolabs , P6010S.

    Article Title: Extraction of mRNA from stalled ribosomes by the Ski complex
    Article Snippet: Casein Kinase II , New England Biolabs , P6010S.


    Recombinant:

    Article Title: Casein Kinase II Phosphorylation of Spt6 Enforces Transcriptional Fidelity by Maintaining Spn1-Spt6 Interaction
    Article Snippet: .. Recombinant Casein Kinase II , New England Biolabs , P6010S. .. ATP, [γ-32P]-6000Ci/mmol, 10mCi/ml, , Perkin Elmer , BLU002Z500UC.



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    Pv CK2α and Pv PP2AC regulate Ser548 dephosphorylation and hemocyanin degradation (A) Validation of p-S548 antibody using recombinant Pv HMCs-Flag (WT) and Ser548 mutants treated with HsCK2α or Pv PP2AC-GST. (B) Western blot of phosphorylation and dephosphorylation states of Pv HMCs and Ser548 mutants after trypsin digestion. (C and D) Western blot of hemocyanin degradation peptides and Ser548 phosphorylation following dual knockdown of Pv CK2α + Pv Trypsin (C) or Pv PP2A + Pv Trypsin (D) in P. vannamei hepatopancreas. Data are representative of ≥2 independent experiments, pooled samples from six shrimp per group.
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    Pv CK2α and Pv PP2AC regulate Ser548 dephosphorylation and hemocyanin degradation (A) Validation of p-S548 antibody using recombinant Pv HMCs-Flag (WT) and Ser548 mutants treated with HsCK2α or Pv PP2AC-GST. (B) Western blot of phosphorylation and dephosphorylation states of Pv HMCs and Ser548 mutants after trypsin digestion. (C and D) Western blot of hemocyanin degradation peptides and Ser548 phosphorylation following dual knockdown of Pv CK2α + Pv Trypsin (C) or Pv PP2A + Pv Trypsin (D) in P. vannamei hepatopancreas. Data are representative of ≥2 independent experiments, pooled samples from six shrimp per group.
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    Pv CK2α and Pv PP2AC regulate Ser548 dephosphorylation and hemocyanin degradation (A) Validation of p-S548 antibody using recombinant Pv HMCs-Flag (WT) and Ser548 mutants treated with HsCK2α or Pv PP2AC-GST. (B) Western blot of phosphorylation and dephosphorylation states of Pv HMCs and Ser548 mutants after trypsin digestion. (C and D) Western blot of hemocyanin degradation peptides and Ser548 phosphorylation following dual knockdown of Pv CK2α + Pv Trypsin (C) or Pv PP2A + Pv Trypsin (D) in P. vannamei hepatopancreas. Data are representative of ≥2 independent experiments, pooled samples from six shrimp per group.
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    Pv CK2α and Pv PP2AC regulate Ser548 dephosphorylation and hemocyanin degradation (A) Validation of p-S548 antibody using recombinant Pv HMCs-Flag (WT) and Ser548 mutants treated with HsCK2α or Pv PP2AC-GST. (B) Western blot of phosphorylation and dephosphorylation states of Pv HMCs and Ser548 mutants after trypsin digestion. (C and D) Western blot of hemocyanin degradation peptides and Ser548 phosphorylation following dual knockdown of Pv CK2α + Pv Trypsin (C) or Pv PP2A + Pv Trypsin (D) in P. vannamei hepatopancreas. Data are representative of ≥2 independent experiments, pooled samples from six shrimp per group.
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    Image Search Results


    Pv CK2α and Pv PP2AC regulate Ser548 dephosphorylation and hemocyanin degradation (A) Validation of p-S548 antibody using recombinant Pv HMCs-Flag (WT) and Ser548 mutants treated with HsCK2α or Pv PP2AC-GST. (B) Western blot of phosphorylation and dephosphorylation states of Pv HMCs and Ser548 mutants after trypsin digestion. (C and D) Western blot of hemocyanin degradation peptides and Ser548 phosphorylation following dual knockdown of Pv CK2α + Pv Trypsin (C) or Pv PP2A + Pv Trypsin (D) in P. vannamei hepatopancreas. Data are representative of ≥2 independent experiments, pooled samples from six shrimp per group.

    Journal: iScience

    Article Title: Dephosphorylation at Ser548 regulates hemocyanin-derived antibacterial peptides and immune defense in Penaeus vannamei

    doi: 10.1016/j.isci.2026.115119

    Figure Lengend Snippet: Pv CK2α and Pv PP2AC regulate Ser548 dephosphorylation and hemocyanin degradation (A) Validation of p-S548 antibody using recombinant Pv HMCs-Flag (WT) and Ser548 mutants treated with HsCK2α or Pv PP2AC-GST. (B) Western blot of phosphorylation and dephosphorylation states of Pv HMCs and Ser548 mutants after trypsin digestion. (C and D) Western blot of hemocyanin degradation peptides and Ser548 phosphorylation following dual knockdown of Pv CK2α + Pv Trypsin (C) or Pv PP2A + Pv Trypsin (D) in P. vannamei hepatopancreas. Data are representative of ≥2 independent experiments, pooled samples from six shrimp per group.

    Article Snippet: For phosphorylation, dephosphorylated Pv HMC (1 mg) was incubated at 30°C for 6 h with human CK2 (HsCK2, New England Biolabs, P6010S) in a reaction containing 10× NEB buffer (50 mM Tris-HCl, 10 mM MgCl 2 , 0.1 mM EDTA, 2 mM DTT, 0.01% Brij 35, pH 7.5), 0.5 μL HsCK2, and 10 μL ATP (New England Biolabs, P0756S).

    Techniques: De-Phosphorylation Assay, Biomarker Discovery, Recombinant, Western Blot, Phospho-proteomics, Knockdown

    Pv CK2α and Pv PP2AC regulate Ser548 dephosphorylation and hemocyanin degradation (A) Validation of p-S548 antibody using recombinant Pv HMCs-Flag (WT) and Ser548 mutants treated with HsCK2α or Pv PP2AC-GST. (B) Western blot of phosphorylation and dephosphorylation states of Pv HMCs and Ser548 mutants after trypsin digestion. (C and D) Western blot of hemocyanin degradation peptides and Ser548 phosphorylation following dual knockdown of Pv CK2α + Pv Trypsin (C) or Pv PP2A + Pv Trypsin (D) in P. vannamei hepatopancreas. Data are representative of ≥2 independent experiments, pooled samples from six shrimp per group.

    Journal: iScience

    Article Title: Dephosphorylation at Ser548 regulates hemocyanin-derived antibacterial peptides and immune defense in Penaeus vannamei

    doi: 10.1016/j.isci.2026.115119

    Figure Lengend Snippet: Pv CK2α and Pv PP2AC regulate Ser548 dephosphorylation and hemocyanin degradation (A) Validation of p-S548 antibody using recombinant Pv HMCs-Flag (WT) and Ser548 mutants treated with HsCK2α or Pv PP2AC-GST. (B) Western blot of phosphorylation and dephosphorylation states of Pv HMCs and Ser548 mutants after trypsin digestion. (C and D) Western blot of hemocyanin degradation peptides and Ser548 phosphorylation following dual knockdown of Pv CK2α + Pv Trypsin (C) or Pv PP2A + Pv Trypsin (D) in P. vannamei hepatopancreas. Data are representative of ≥2 independent experiments, pooled samples from six shrimp per group.

    Article Snippet: Hs CK2 , NEB , Cat# P6010S.

    Techniques: De-Phosphorylation Assay, Biomarker Discovery, Recombinant, Western Blot, Phospho-proteomics, Knockdown